Master Thesis: Enzyme Catalysis at High Pressure

Betreuer/in:            Marlene Schmalle           
Dekanat/Institut:   Institut für Technische Biokatalyse           

E-Mail:   Marlene.Schmalle@tuhh.de

Project Work: Enzyme Catalysis at High Pressure

Lipases are the most frequently used enzymes in industry and therefore are of great interest. They catalyze a variety of reactions and can be used under a wide range of conditions. One way to optimize these enzymes for industrial applications is to use immobilized enzymes in a continuous reactor. A new field of application is the continuous enzymatic high-pressure synthesis. Below 200 MPa several enzymes show a pressure induced change of their structure that affects their performance in terms of activity, stability and selectivity.

A detailed kinetic study of enzymes from different enzyme classes will help to understand enzyme class specific behaviour under pressure as a complementary process parameter. For this purpose, a continuously
operating high-pressure reactor was designed using immobilized enzymes to investigate enzyme kinetics and the influence of pressure.

Aim
In this work enzyme kinetics will be determined in a novel conceptualized high-pressure reactor to
understand pressure induced changes in enzyme performance. The gained knowledge can be used to determine optimal reaction conditions and to improve the enzymatic reaction system.

Requirements
The work includes a large practical laboratory part and is therefore more suitable for students who enjoy laboratory work and have good practical experience. Students are welcome to contribute their own ideas.

Start: immediately

Contact
Marlene Schmalle M.Sc.
Institut für Technische Biokatalyse
Denickestr. 15 (K), Raum:1514
Marlene.Schmalle@tuhh.de
Tel. +49 40-42878-4265

Schreibe einen Kommentar

Deine E-Mail-Adresse wird nicht veröffentlicht. Erforderliche Felder sind mit * markiert