Publications Prof. Volker Kasche | Institute of Technical Biocatalysis

Publications in journals

2005

S. C. Viegas, D. Schmidt, V. Kasche, C. M. Arraiano, Z. Ignatova
Effect of the increased stability of the penicillin amidase mRNA on the protein expression levels
FEBS Lett., 579, 5069-5073 (2005)
Z. Ignatova, F. Wischnewski, H. Notbohm, V. Kasche
Pro-sequence and Ca2+-binding: Implications for folding and maturatiopn of Ntn-hydrolase Penicillin amidase from E. coli
J.Mol. Biol. 348, 999-1014 (2005)
V. Kasche, Z. Ignatova, H. Märkl, W. Plate, N. Punckt, D. Schmidt, K. Wiegandt, B. Ernst
Ca2+ is a cofactor required for membrane transport and maturation and is a yield determining factor in high cell density penicillin amidase production.
Biotechnol. Prog. 21, 432-438 (2005)

2004

D. BOZHINOVA, B. GALUNSKY, G. YUEPING, M. FRANZREB, R. KÖSTER, V. KASCHE
Evaluation of magnetic polymer micro-beads as carriers of immobilised biocatalysts for selective and stereoselective transformations
Biotechnology Letters, 26, 343-350 (2004)

2003

V. Kasche, Boris Galunsky, Z. Ignatova
Fragments of Pro-peptide Activate Mature Penicillin Amidase of Alcaligenes faecalis
Eur.J.Biochem. 270, 4721-4728 (2003)
V. KASCHE, M. DE BOER, C. LAZO, M. GAD
Direct observation of intraparticle equilibration and the rate limiting step in adsorption of proteins in chromatographic adsorbents with confocal laser scanning microscopy (CLSM)
J. Chromatog. B 790, 115-130 (2003)
Z. Ignatova, A. Mahsunah, M. Georgieva, V. Kasche
Improvement of Posttranslational Bottlenecks in the Production of Penicillin Amidase in Recombinant Escherichia coli strains
Appl.Environ.Microbiol. 69, 1237-1245,(2003)

2002

B. GALUNSKY, V. KASCHE
Determination of the Enantioselectivity for Kinetically Controlled Condensations Catalysed by Amidases and Peptidases
ADV.SYNTH.CATAL. 344, 1115-1119(2002)
Z. IGNATOVA, C. HÖRNLE, A. NURK, V. KASCHE
Unusual Signal Peptide Ddirects Penicillin Amidase from Escherichia coli to the Tat Translocation Machinery
Biochem.Biophys.Res.Commun. 291, 146-149 (2002)

2001

V. KASCHE
Physicochemical boundaries in the continuous and one-step discontinuous affinity-chromatographic isolation of proteins
J. biochem biophys. methods, 49, 49-62 (2001)
A. SPIESS, V. KASCHE
Enzymatic synthesis of amoxicillin. Process integration using multiphase systems
Novel Frontiers in the Production of Compounds for Biomedical Use (A. van Broekhoven et al. eds) Kluwer Academic Publishers, Amsterdam, 169-192 (2001)
A.C. CHICERE, B. GALUNSKY, V. SCHÜNEMANN AND V. KASCHE
Stability of immobilised soybean lipoxygenases: infulence of coupling conditions on the ionisation state of the active site Fe
Enzyme Microb. Technol, 28, 168-175 (2001)
V. KASCHE
Proteases in Peptide synthesis
Proteases a practical approach (I. Bond and R. Beynon, eds.) 2nd edition, Oxford Univ. Press, Oxford, 265-292 (2001)
A. SPIESS, V. KASCHE
Direct measurement of pH profiles in immobilised enzymes during kinetically controlled synthesis using CLSM
Biotechnol Progress., 17, 294-303 (2001)
B. MAHRO, R. MÜLLER, V. KASCHE
Bioavailability ï¿½ the Key Factor of Soil Bioremediation
Springer Verlag Berlin Heidelberg, 181-195, (2001)
V. KASCHE, A. SPIESS
Enzymatic Transformations in suspensions
Enzymes in Nonaqueous Media (P. Halling, B. Holland, E. Vulfson and J. Woodley (eds).) (2001)

2000

A.J.J. Straathof, V. Kasche
Process conceptions for bioconversions
Applied Biocatalysis, harwood academic publishers, in press, (2000)
L. HEWITT, V. KASCHE, K. LUMMER, R.J. LEWIS, G.N. MURSHUDOV, G.G. DODSON AND K.W. WILSON
Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active site cleft
J. Mol. Biol., 302, 887-898 (2000)
Z. IGNATOVA, S. TARUTTIS, V. KASCHE
Role of the intracellular proteolysis in the production of the periplasmtic penicillin amidase in Escherichia coli
Biotechnol. Letters, 22, 1727-1732 (2000)
A.C. CHIKERE, B. GALUNSKY, A. OVERMEYER, G. BRUNNER, V. KASCHE
Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media
Biotechnol. Letters 22, 1815-1821 (2000)
BORIS GALUNSKY, KARSTEN LUMMER, VOLKER KASCHE
Comparative Study of Substrate- and Stereospecificty of Penicillin G Amidases from Different Sources and Hybrid Isoenzymes
Chem. MONTHLY, 131, 623-632 (2000)
Z. IGNATOVA, S.-O. ENFORS, S. HOBBIE, S. TARUTTIS, C. VOGT, V. KASCHE
The relative importance of intracellular proteolysis and transport on the yield of the periplasmatic enzyme penicillin amidase in ESCHERICHIA COLI
Enzyme Microb. Technol., 26 165-170 (2000)

-1999

V. KASCHE, K. LUMMER, A. NURK, E. PIOTRASCHKE, A. RIEKS, S. STOEVA, W. VOELTER
Intramolecular autoproteolysis initiates the maturation of penicillin amidase from E. coli
Biochem. Biophys. Acta, 1433, 76-86 (1999)
K. LUMMER, A. RIECKS, B. GALUNSKY, V. KASCHE
pH-dependence of penicillin amidase enantioselectivity for charged substrates
Biochem. Biophys. Acta, 1433, 327-334 (1999)
L. HEWITT, V. KASCHE, K. LUMMER, A. RIEKS, K. WILSON
Crystallization of a precursor penicillin acylase from Escherichia coli
Acta. Crystallog., 55, 1052-1054, (1999)
W. TISCHER, V. KASCHE
Immobilized Enzymes: Crystals or Carriers?
TIBTECH, 17, 326-335, (1999)
A. OVERMEIER, S. SCHRADER-LIPPELT, V. KASCHE, G. BRUNNER
Lipase-catalysed kinetic resolution of racemates at temperatures from 400C to 1600 in supercritical carbon dioxide
Biotechnol. Letters, 21, 65-69 (1999)
K. LÜDTKE, K.-V. PEINEMANN, V. KASCHE, R.-D. BEHLING
Nitrate removal of drinking water by means of catalytically active membranes
J. Membrane Science, 151, 3-12, (1999)
A.SPIESS, R.SCHLOTHAUER, J.HINRICHS, B.SCHEIDAT, V. KASCHE
pH gradients in heterogeneous biocatalysts and their influence on rates and yields of the catalysed processes
Biotechnol. Bioeng., 62, 267-277 (1999)
ANTHONY CHIKERE, BORIS GALUNSKY, VOLKER KASCHE
Soybean Lipopxygenases: Purification and Stability of the Free and Immobilized Enzymes
Progress in Biotechnology (A. Ballesteros, F.J. Plou, J.L. Iborra., P.J. Halling (eds) Elsevier Science, Amsterdam, 15, 559-564 (1998)
Z. IGNATOVA, S. STOEVA, B. GALUNSKY, C. HÖRNLE, A. NURK, E. PIOTRASCHKE, W. VOLTER, V. KASCHE
Proteolytic processing of penicillin amidase from Alcaligenes faecalis cloned in E. coli yields several active forms
Biotechnology Letters, 20, 977-982 (1998)
B. GALUNSKY, S. IGNATOVA. V. KASCHE
Temperature effects on S1- and S1`-enantionselectivity of alpha-Chymotrypsin
Biochem. Biophys. Acta. 1343, 130-138 (1997)
N. GOTTSCHLICH, V. KASCHE
Purification of monoclonal antibodies by simulated moving-bed chromatography
J. Chromatog.765, 201-206 (1997)
I. Ivanov, V. KASCHE, D. PETKOV
Thermodynamics of Enzymic Peptide Synthesis in Biphasic Aqueous-Organic Systems: Coupling of the Chemical and Phase Equilibria
Biocatalysis and Biotransformation 14, 181-194 (1997)
B. MAHRO, A. ESCHENBACH, L. LEHNE, G. SCHAEFER, R. WIENBERG, V. KASCHE
Möglichkeiten und Grenzen zur Beeinflussung des biologischen PAK-Abbaus
Neue Techniken der Bodenreinigung (Re. Stegmann) Economica Verlag, p. 317-329 (1996)
O. GUTHMANN, R. SCHWERDTFEGER, A. RIEKS, G. ANTRANIKIAN, V. KASCHE, G. BRUNNER
Enzyme catalysed reactions, enantioselectivity and stability under high hydrostatic pressure
High Pressure Chemical Engineering (Ph. R. von Rohr und Ch. Trepp, Hrsg.) Elsevier Science, 127-131 (1996)
V. KASCHE, B. GALUNSKY, A. NURK, E. PIOTRASCHKE, A. RIEKS
The dependeny of the stereoselectivity of penicillin amidases – enzymes with R-specific S1- and S-specific Sï¿½1-subsites- on temperature and pimary structure
Biotechnol. Letters, 18, 455-460 (1996)
N. GOTTSCHLICH, S. WEIDGEN, V. KASCHE
Continuous biospecific affinity purification of enzymes by simulated moving bed chromatography: Theoretical description and experimental results
J. Chromatog. 719, 267-274 (1996)
V. KASCHE
An integrated approach in the analytical description of affinity chromatography, biosensors, immobilized biocatalysts and similar systems
Adv. in Mol. Cell. Biol. 15.B 605-620 (1996)
B. MAHRO, K. ROHDE, V. KASCHE
Non-Selective Precultivation of Bacteria Able to Degrade Different Polycyclic Aromatic Hydrocarbons (PAH)
Acta Biotechnol. 15, 337-345 (1995)
M. KLINGEBERG, B. GALUNSKY, C. SJÖHOLM, V. KASCHE, G. ANTRANIKIAN
Purification and properties of highly thermostable, SDS resistant and stereospecific proteinase from the extreme thermophilic archeon Thermococcus stetteri
Appl. environm. Microbiol., 61, 3098-3104 (1995)
V. KASCHE, B. GALUNSKY
Enzyme catalyzed biotransformations in aqueous two-phase systems with precipitated substrate and/or product
Biotechnol. Bioeng. 45, 261-267 (1995)
V. Kasche, G. Schietke, J. Behnert, H.-J. Thes Probenaufarbeitungssystem für Bio- und Umwelttechnik
BIOforum, 17, 138-140 (1994)
B. GALUNSKY, R. SCHLOTHAUER, B.BÖCKLE, V.KASCHE
Direct spectrometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme
Anal. Biochem. 221, 213-214 (1994)
E. PIOTRASCHKE, A. NURK, B. GALUNSKY, V. KASCHE
Genetic construction of catalytically active cross-species heterodimer penicillin G amidase
Biotechnol. Letters 16, 119-124 (1994)
O.C. AWORH, V. KASCHE,. and O.O. APAMPA
Purification and some properties of Sodom Apple Latex Proteinases
Food Chem. 50, 359-362 (1994)
V. KASCHE, N. GOTTSCHLICH, A. LINDBERG, C. NIEBUHR- REDDER, AND J. SCHMIEDING
Perfusible and non-perfusible supports with monoclonal antibodies for biospecic purification of E.coli penicillin amidase within its pH-stability range
J.Chromatog.660, 137-145 (1994)
A. BALLESTEROS, K. BUCHHOLZ, L. BOROSS, J. CABRAL and V. KASCHE
Biocatalyst performance
Applied Biocatalysis (J. Cabral, D. Best, L. Boross, J. Tramper, eds.), Chapt. 5, Harwood academic publishers, Chur, Kap. 5 (1994)
A. KÄÄRD, A. SCHWELLENBACH, V. KASCHE, E. RENKEN
A fluorescence based penicillin biosensor for on-line monitoring of benzylpenicillin hydrolysis. Optimization of the fluorescence signal for the measurement of benzylpenicillin
J.Anal.Chim. (Russ.Akad.Wiss.) 48, 306-312 (1993)
M.A. DINEVA, B. GALUNSKI, V. KASCHE, and D. PETKOV
Phenylacetyl group as enzyme-cleavable aminoprotection of purine nucleotides
Bioorg.Medic.Chem Letters, 3, 2781-2784 (1993)
V. KASCHE, W. HAß, U. HAUFLER, G. MICHAELIS, C. NIEBUHR-REDDER, Th. SCHOLZEN, and T. WIESEMANN
Penicillin Amidase from E.coli
50 years of penicillin application (H. Kleinkauf, and H.v. Döhren, eds.) Public Ltd, Prague, 291-303 (1993)
E.K. BRATOVANOVA, V. KASCHE, D.D. PETKOV
Kinetically-controlled enzymic synthesis of ribonucleotide bond in vitro
Biotechnol. Letters 15, 347-352 (1993)
O.C. AWORH and V. KASCHE
Purification an preliminary characterization of sodom apple proteinase
DECHEMA Biotechnology Conferences 5, 137-140 (1992)
V.KASCHE, W. HAß, C. NIEBUHR-REDDER, and E. PIOTRASCHKE
Penicillin Amidase aus E.coli – ein biotechnisches Enzym auf der Suche nach einer biologischen Funktion
BioEngineering 8, 69-77 (1992)
A. BOHMANN, R. PÖRTNER, J. SCHMIEDING, V. KASCHE AND H. MÄRKL
The membrane dialysis bioreactor with integrate radial-flow fixed bed – a new approach for continuous cultivation of animal cells
Cytotechnology 9, 51-57 (1992)
B. MAHRO, A. PETERMANN-HERMANN, S. WALENTA, W. MÜLLER-KLIESER, and V. KASCHE
Non-extractive localization and imaging of luminescent bacteria in liquid and soil samples by luminescence microscopy
Microb. Releases 1, 79-85 (1992)
A.NADIM, I.B.STOINEVA, B.GALUNSKY, V. KASCHE and D. PETKOV
Mass transfer induced interchange of the kinetic and thermodynamic control of enzymic peptide synthesis in biphasic water-organic systems
Biotechnology Techniques 6, 539-544 (1992)
M. SCHUSTER, V. KASCHE, H.-D. JAKUBKE
Contribution to the Sï¿½-subsite specificity of papain
Biochem.Biophys.Acta, 1121, 207-212 (1992)
V. KASCHE, E.RENKEN, G, SCHIETKE, R. ULRICH, R. BÜCKE, H. GNEWUCH, K. JANSEN
Signal generation and evaluation in fluorescence based on-line fiber optic biosensors
Biosensors: Fundamentals, Technologies and Applications (F. Scheller, R.D. Schmid, eds.) VCH Verlagsgesellschaft mbH, Weinheim p. 265-274 (1992)
LESLEY J. BARTUNIK, HANS D. BARTUNIK, LOTHAR R. JACOB, VOLKER KASCHE AND THOMAS WIESEMANN
Crystallization and preliminary x-ray data of proteolitically processes penicillin amidase PA 6.7 from Escherichia Coli
(1991)
M. Schuster, H.-D. Jakubke and V. Kasche
Nucleophile specificity in papain-catalyzed acyl transfer reactions
Biomed.Biochim.Acta 50, 12-126 (1991)
C. NIEBUHR-REDDER, V. KASCHE
Antibodies to E. coli Penicillin G Amidase for biochemical studies and the control of fermentation processes
Proceedings of the German-Japanese Workshop on animal cell culture technology, December 4-5 1990, Japan Society for the Promotion of Science, 57-60 (1991)
V. KASCHE, G. MICHAELIS, T. WIESEMANN
Stereo-and sequence specificity of serine proteases in peptide synthesis
Biomed. Biophys. Acta 50, 38-43 (1991)
V. KASCHE, B. GALUNSKY, and G. MICHAELIS
Binding of organic solvent molecules influences the P1′-P2′ stereo and sequence specificity of a-chymotrypsin in kinetically controlled peptide synthesis
Biotechnology Letters 13, 75-80 (1991)
B. MAHRO, L. SCHLEINKOFER, V. KASCHE
In situ detection and localization of bioluminescent Streptomyces coelicolore in liquid and soil by photon imaging
Acta. Biotechnol. 11, 541-546 (1991).
V: SCHELLENBERGER, H.-D. JAKUBKE, and V.KASCHE
Electrostatic effects in the alfa-chymotrypsin-catalyzed acyl transfer II. Efficiency of nucleophiles bearing charged groups in various locations
Biochem.Biophys.Acta 1078, 8-11 (1991).
A. Käärd, V. Kasche, E. Renken and G. Schietke
FLUORESCENCE BASED PH BIOSENSOR FOR ON-LINE MONITORING OF BENZYLPENICILLIN-HYDROYLSIS (IN RUSSIAN)
E. RENKEN, A. KÄÄRD, and V. KASCHE
Fluorescence of proteins adsorbed to chromatographic supports: Application in studies on adsorption – desorption kinetics
Fundamentals of Adsorption (A.B. Mersmann, S.E. Schol, eds.) United Eng. Trustees Inc, New York, p. 715-722 (1991)
M. KÄSTNER, B. MAHRO, V. KASCHE
Biologische Abbaubarkeit von Schadstoffen
Reinigung kontaminierter Böden (R. Stegmann, V. Franzius eds.) Economica Verlag, p. 27-47 (1990)
G.MICHAELIS, and V. KASCHE
S1′- subsite mapping of penicillin amidase in the kinetically controlled peptide synthesis
DECHEMA Biotechnology Conferences, 4, 293 (1990)
E. RENKEN, A. KÄÄRD, G. SCHIETKE, and V. KASCHE
Automatic online monitoring of benzylpenicillin hydrolysis with a fluorescnec-based penicillin biosensor
DECHEMA Biotechnology Conferences, 4, 949 (1990)
V. KASCHE, and R. ULRICH
Fluorescence Biosensors: Signal generation and signal transmission with fiber optics
Fresenius J. Anal. Chem. 337, 24-25 (1990)
V. KASCHE, T. SCHOLZEN, TH. BOLLER, D.M. KRÄMER, F. LÖFFLER
Rapid Protein Purification using PBA-EUPERGITTM a novel method for Large-Scale Procedures
J. Chromatog. 510, 149-154 (1990)
E. RENKEN, V. KASCHE, A. KÄÄRD
Optimization of the Signals of Biosensors based on pH dependent Fluorescence Changes
DECHEMA Biotechnology Conferences Vol. 3, 743-746 (1990)
B. MAHRO, V. KASCHE, L. SCHLEINKOFER
Photon Imaging: A powerful tool for the environmental monitoring of genetically engineered micoroorganisms
DECHEMA Biotechnology Conferences Vol. 3, Frankfurt, 447-452 (1990)
V. KASCHE, G. MICHAELIS
Protease catalysed peptide synthesis in systems with organic solvents
DECHEMA Biotechnology Conferences Vol. 3, 237-240 (1990)
V. KASCHE und G. MICHAELIS
Peptide Bond Synthesis by Enzyme-Catalyzed Acyl-Transfer
in: Biochemistry of Peptide Antibiotics, (Kleinkauf and v.Döhren, eds.), W. de Gruyter, Berlin, 81-101 (1990) 81. VOLKER KASCHE, GUNTER SCHIETKE, JÜRGEN BEHNERT UND KURT-PETER RAEZKE Prozessüberwachung und-steuerung von Bioreaktoren. In BioTec, Juli 1990
E. Renken, A. Käärd, and V. Kasche
Fluorescence of Proteins Adsorbed to chromatographic supports: Application in studies on adsorption-desorption kinetics
Fundamentals of Adsorption. Eng.Formel.Conf. May 1989
V. KASCHE and E. RENKEN
Biosensoren – Sensoren fÜr die Biotechnik
Chem.-Ing.-Tech. 61,.783-787, (1989)
V. KASCHE, A. KÄÄRD, E. RENKEN, H. GREWE, K. JANSEN and R. ULRICH
DYNAMIC fluorescence biosensors: Principles of signal generation and of coupling to optical-fibers
Biosensors: application in medicine, environmental protection, and process control, (R. D. Schmid and F. Scheller. eds.), Weinheim, Basel, Cambridge, New York,) p. 233-242 (1989)
J. DITTMER, A. DITTMER, R. DELLA BRUNA, V. KASCHE
A native, affinity-based protein blot for the analysis of streptavidin heterogeneity: Consequences for the specificity of streptavidin mediated bindung assays
Electrophoresis, 10, 762-765 (1989)
V. KASCHE
Proteases in peptide synthesis
Proteases a practical approach( R. Beynon, and J. Bond eds.) IRL Press, Oxford, 125-143 (1989)
A. SCHÖNE, M. ZIERUL, V. KASCHE
Simulation and model-based prozess control of continuously operated fermentation processes for the production of microbial metabolites
DECHEMA-BIOTECHNOLOGY-CONFERENCES vol. 2, VCH, Weinheim Frankfurt, in press, 117-130 (1988)
V. KASCHE, R. SCHLOTHAUER and G. BRUNNER
Enzyme denaturation in supercritical CO2: Stabilizing effect of S-S bonds during the depressurization step
Biotechnol. Letters, 10, 569-574 (1988)
U. HAUFLER, R. ULMKE, T. WIESEMANN, and V. Kasche
Structure, pH-stability and renaturation of free and immobilized E. coli penicillin Amidase
Biotechnology in Germany (H.J. Rehm, ed.) Verlag Chemie, Weinheim, 345-350 (1988)
V. KASCHE
Hydrolases as biocatalysts for the synthesis of biologically active condensation products (b-lactam antibiotics and peptides)
Biotechnology of biologically active natural products (R. Vlakov, ed.) Bulgarian Academy of Science
V. KASCHE, B. GALUNSKY, and D. D. PETKOV
Biotechnologische Grundlagen der enzymkatalysierten Synthese von Kondensationsprodukten
Isvestija po chimia, 20, 192 (1987)
V. KASCHE
Mechanisms and maximum yield in kinetically controlled peptide synthesis
Advances in the Biosciences, 65, 151-158 (1987).
V. KASCHE, U. HAUFLER, and L. RIECHMANN
Equilibrium and Kinetically Controlled Synthesis with Enzymes: Semisynthesis of Penicillins and Peptides
Methods in Enzymology, 136, 280-292 (1987)
V. KASCHE, U. HAUFLER, D. MARKOWSKY, S. MELNYK, and A. ZEICH
Penicillin Amidase from E. coli-enzyme heterogeneity and stability
Ann. N.Y. Acad. Sci., 501, 97-102 (1987)
V. KASCHE
Mechanism and yields in enzyme catalyzed equilibrium and kinetically controlled synthesis of ï¿½-lactam antibiotics peptides and other condensation products
Enzyme Microb. Technol., 8, 4-16 (1986)
L. RIECHMANN, and V. KASCHE
Reaction mechanism, specificity and pH dependence of peptide synthesis catalyzed by the metalloproteinase thermolysin
Biochim. Biophys. Acta, 872, 269-276 (1986)
J. BULLERDIEK, J. DITTMER, A. FAEHRE, S. BARTNITZKE, V. KASCHE, and W. SCHLOOT
A new banding pattern of human chromosomes after nick-translation using eco RI and biotin-dUTP
Clin. Genet., 28, 173-178 (1985)
V. KASCHE
Ampicillin and cephalexin synthesis catalyzed by E. coli penicillin amidase yield increase due to substrate recycling
Biotechnol. Letters 7, 877-882 (1985)
L. RIECHMANN, and V. KASCHE
Peptide synthesis catalyzed by the serine proteases chymotrypsin and trypsin
Biochem. Biophys. Acta., 830, 164-172 (1985)
V. KASCHE, and U. HAUFLER
Enzyme catalyzed semisynthesis of condensation products – kinetic vs. equilibrium control
Third European Congress of Biotechnology, Verlag Chemie, Weinheim, 425-434 (1984)
V. KASCHE, U. HAUFLER, and R. Zï¿½LLNER
Kinetic studies on the mechanism of the penicillin amidase catalyzed synthesis of ampicillin and benzylpenicillin
Hoppe Seyler’s Z. Physiol. Chem., 365, 1435-1443 (1984)
V. KASCHE, and U. HAUFLER
Ausbeutebestimmende Faktoren bei der enzymkatalysierten Synthese von semisynthetischen beta-Lactamamantibiotica
DECHEMA Monographs, Vol. 95, Verlag Chemie, Weinheim, S. 33-49 (1984)
V. KASCHE, U. HAUFLER, l. RIECHMANN, und R. ZÖllner
Kinetically controlled semisynthesis of beta-lactam antibiotics and peptides
Ann. N.Y. Acad. Sci., 434, 99-105 (1984)
L. RIECHMANN, and V. KASCHE
Kinetic studies on the mechanism and specificity of peptide semisynthesis catalyzed by the serine proteases alpha-chymotrypsin and beta-trypsin
Biochem. Biophys. Res. Comm., 120, 686-691 (1984)
L. RIECHMANN, and V. KASCHE
Sequence specificity in proteinase-catalyzed peptide semi-synthesis – does it reflect substrate specificity
Hoppe Seyler’s Z. Physiol. Chem., 364, 1200 (1983)
V. KASCHE, B. GALUNSKY, U. HAUFLER, and R. ZÖLLNER
Kinetically vs. equlibrium-controlled synthesis of C-N bonds in beta-lactams and peptides with free and imobilized biocatalysts
Enzyme Technology (R.M. Lafferty, ed.), Springer Verlag, 297-308 (1983)
V. KASCHE
Correlation of theoretical and experimental data for immobilized biocatalysts
Enzyme Microbiol. Technol., 5, 2-13 (1983)
R. AGUIRRE, and V. KASCHE
Catalytically active monomer forms of immobilized arginase
Eur.J.Biochem., 130, 373-381 (1983)
P. ZIPFEL, J. DITTMER, V. KASCHE, and H. AMNEUS
DNA-turnover in cultured animal cells
Hoppe Seyler’s Z. Physiol. Chem., 363, 937 (1982)
V. KASCHE, and R. ZÖLLNER
Tris (hydroxylmethyl) methylamine is acylated when it reacts with acyl-chymotrypsin
Hoppe Seyler’s Z. Physiol. Chem., 363, 531-534 (1982)
K. BUCHHOLZ, A. BORCHERT and V. KASCHE
Aspects of affinity chromatography in HPLC
Practical Aspects of Modern High Performance Liquid Chromatography (J. Molnar, ed.) Walter de Gruyter, Berlin, New York, 187-196 (1982)
V. KASCHE, and B. GALUNSKY
Ionic strength and pH-effects in the kinetically controlled synthesis of bezylpenicillin by nuleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-Aminopenicillanic adid
Biophys. Biochem. Res. Comm., 104, 1215-1222 (1982)
V. KASCHE, and B. GALUNSKY
Kinetically controlled synthesis via acyl-enzyme intermediates using free and immobilized hydrolases
Biotechnology of Biologically Active Natural Products (R.Vlahov, ed.) Bulgarian Academy of Science, Sofia (1982)
V. KASCHE, and B. GALUNSKY
Ligand/Ligate interactions in heterogeneous systems: Their influence on operational properties of affinity chromatographic and binding assay adsorbents
Affinity Chromatography IV (T. Gribnau and J. Visser, eds.), Elsevier-North, Holland, Publ. Co, Amsterdam (1982)
V. KASCHE, B. GALUNSKY and K. BUCHHOLZ
Resolution in high performance liquid affinity chromatography. Dependence on eluite diffusion into the stationary phase
J. Chromatogr., 216, 169-174 (1981)
V. KASCHE, and I. BÜCHTMANN
A method for the isolation of homogeneous fluoresceinla-belled proteins for studies on the variation in biological and fluorescence properties of the different konjugates
Hoppe Seyler’s Z. Physiol. Chem., 362, 1007-1012 (1981)
V. KASCHE, R. Zï¿½LLNER, H. AMNEUS, and L. NÄSLUND
A two-step procedure for quantitative isolation of pure double strand DNA from animal tissue and cell cultures
Prep. Biochem. 11, 233-251 (1981)
V. KASCHE
Stimulus-secretion coupling as an effector mediated self organisation process involving membrane fusion
Self-organizing Systems (G. Roth and H. Schwegler, eds.) Campus Verlag, Frankfurt, 73-83 (1981)
V. KASCHE, K. PROBST, and J. MAASS
The DNA, RNA and trypsin content in trypsins used in monolayer animal cell culture studies
Cell Biol. Int. Reports, 5 , 1 (1981)
V. KASCHE, and I. BÜCHTMANN
Purification of flurescein-labelled chymotrypsin by isoelectric focussing after biospecific adsorption to soybean trypsin inhibitor
Structure and Activity of Natural Peptides (W. Voelter and G. Weitzel, eds.) Walter de Gryter Verlag, Berlin, 273-286 (1981)
V. KASCHE, and G. KUHLMANN
Direct measurements of the thickness of the unstirred diffusion layer outside immobilized biocatalysts
Enzyme Microbiol. Technol., 2 , 309-312 (1980)
V. KASCHE, and P. ZIPFEL
Die spezifische Aktivität von Tritium und C-14 in DNA in Abhängigkeit von der spezifischen Aktivität der organischen und anorganischen Vorstufen in der Nahrung
Strahlenschutzprobleme in Zusammenhang mit der Verwendung von Tritium und Kohlenstoff-14 und ihren Verbindungen (F. Stieve und G. Kistner, Hrsg.). Bundesï¿½gesundheitsamt, STH Berichte 12/1980, 107-111 (1980)
V. KASCHE, and R. LANGENBERG
The differences between plug-flow and continuous backmix reactors with immobilized biocatalysts. Can theoretical calculations predict the experimental behaviour?
Food Process Engineering (P. Linko, Y. MÄlkki, J. Olkku and P. Larinkari, eds.) vol. 2, Applied Science Publ., Barking, England (1980)
V. KASCHE
Characterization of biospecific adsorbents used for affinity chromatography
Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 367-377 (1979)
V. KASCHE, and K. BUCHHOLZ
Mass transfer influence on effectivness
Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 208-243 (1979)
K. BUCHHOLZ, E. EHRENTHAL, M. GLOGER, N. HENNRICH, O. JAWOREK, V. KASCHE, J. KLEIN, D.M. KRÄMER, M.R. KULA, G. MANECKE, D. PALM, J. SCHLöNSEN, and F. WAGNER
Characteristic properties and summary of determination methods
Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 1-48 (1979)
I. BÜCHTMANN, and V. KASCHE
Purification of fluorescein labelled chymotrypsin by isoelectric focussing after biospecific adsorption to soybean trypsin inhibitor
Hoppe Seyler’s Z. Physiol. Chem., 360 , 1136- ? (1979)
R. AGUIRRE, and V. KASCHE
Affinity chromatography of subunit proteins: The reversible dissociation of sepharose-bound rat-liver arginase and its application in subunit exchange chromatography
Hoppe Seyler’s Z. Physiol. Chem., 360 , 222 (1979)
H. AMNEUS, D. GABEL, L. NÄSLUND, and V. KASCHE
Biospecific sample reparation and application in isoelectric focussing
Biochem. Biophys. Res. Comm., 90 , 1313-1320 (1979)
V. KASCHE, A. KAPUNE, and H. SCHWEGLER
The operational effectiveness factor of immobilized enzyme systems
Enzyme Microbiol. Technol., 1 , 41-46 (1979)
V. KASCHE
Prozesse an Membranen – Einflußï¿½ der Mikroumgebung und Massentransports
Zelluläre Kommunikations- und Kontrollmechanismen (L. Rensing und G. Roth, Hrsg.), Universität Bremen, Bremen, 13-26 (1978)
V. KASCHE, R. Zï¿½LLNER, H. AMNEUS, and L. NÄSLUND
A rapid and quantitative method to determine the tritium content in DNA from small tissue samples
in: IAEA/NEA International Symposium on the Behaviour of Tritium in the Environment, Oct. 16-20 1978, International Atomic Energy Agency, Wien, 173-183 (1978)
V. KASCHE, A. KAPUNE, and H. SCHWEGLER
Relative importance of diffusion layer resistance and microenvironmental effects on the effectivness of immo-bilized enzyme reactors
Enzyme Engineering (G. Manecke, G. Brown, L.ï¿½Wingard, eds.), vol. 4, Plenum Press, New York, 365-371 (1978)
V. KASCHE, D. AMNEUS, and D. GABEL
Physical-chemical factors influencing the resolution in affinity chromatography
Enzyme Enginneering (G. Manecke, G. Brown, L. Wingard, eds.), vol. 4, Plenum Press, New York, 449-450 (1978)
A. KAPUNE, and V. KASCHE
Kinetically controlled equilibria: The perturbation of hydrolysis equilibria in reactions catalyzed by alpha-chymotrypsin immobilized on charged supports
Biochem. Biophys. Res., 80 , 955-962 (1978)
H. AMNEUS, V. KASCHE, D. GABEL, and H. LINDQVIST
Affinity chromatography of chymotrypsin on soybean trypsin inhibitor sepharose: Applications in genetics and nucleide labelling
Affinity Chromatography (Ostenhof et al., ed.), London: Butterworth, 99-102 (1977)
V. KASCHE, H. MANNWEILER, and P. ZIPFEL
Dose estimations for tritium and 14C released in the nuclear fuel Cycle. A biological and radio-biological evaluation
Quantifizierbarkeit des Strahlenrisikos (I. Schmitz-Feuerhake, Hrsg.) UniversitÄt Bremen, Bremen (1977)
V. KASCHE, H. AMNEUS, L. NÄSLUND, and R. Zï¿½LLNER
A rapid two-step procedure to isolate native DNA
Hoppe Seyler’s Z. Physiol. Chem., 358 , 256 (1977)
V. KASCHE, H. AMNEUS, and L. NÄSLUND
Rapid zymogen activation and isolation of serine proteases from an individual mouse pancreas by affinity chroma-tography. Genetial heterogeneity of chymotrypsins of mus musculus
Biochim. Biophys. Acta., 490 , l-18 (1977)
D. GABEL, and V. KASCHE
Conformational transitions in immobilized enzymes
Methods of Enzymology (K. Mosbach, ed.) vol. 44, Academic Press, New York, 526 (1976)
V. KASCHE, and A. KAPUNE
Immobilized enzymes – their relevance as model systems for reactions at the cell surface: Influence of diffusion layer thickness on reaction rates
Stud. biophys., 56 , 47-48 (1976)
D. GABEL, H. AMNEUS, and V. KASCHE
Resolution in affinity chromatography
J. Chromatogr., 120 , 391-397 (1976)
V. KASCHE
The kinetics of trypsinogen activation studied by analytical affinity chromatography
Arch. Biochem. Biophys., 173 , 269-272 (1976)
V. KASCHE
Radiation-induced crosslinking of alpha-chymotrypsin to DNA and an agarose gel
Int. J. Rad. Biol., 26 , 455-465 (1974)
V. KASCHE
Oxygen effect: Differences in radiation-induced deactivation of mobile and immobilized enzymes
Rad. Environm. Biophysics, 11 , 189-193 (1974)
V. KASCHE, and M. BERGWALL
Intrinsic molecular properties and inhibition of immobilized enzymes. Theory and experimental observations on alpha-chymotrypsin-Sepharose
Insolubilized Enzymes (M. Salmona, C. Saronio and S. Garattini, eds.) Raven Press, New York, 77-86 (1974)
V. KASCHE
An affinity chromatographic study of trypsinogen activation. Kinetic evidence for the existence of neotrypsinogen Z. Klin. Chem. Klin. Biochem., 12 , 242 (1974)
D. GABEL, and V. KASCHE
Autolysis of beta-trypsin. Influence of calcium ions and heat
Acta Chem. Scand., 27 , 1971-1981 (1973)
V. KASCHE
Effects of the microenviroment on the specific interacti-on between alpha-chymotrypsin and immobilized soybean trypsin inhibitor
Studia Biophysica, 35 , 45-56 (1973)
D. GABEL, and V. KASCHE
Cooperative transitions between active alpha-beta-trypsin conformations
Biochem. Biophys. Res. Comm., 48 , 1011-1018 (1972)
V. KASCHE, H. Linqvist, R. Bergman, and R. AXEN
A theoretical model describing steady state catalysis by enzymes immobilized in spherical gel particles. Experimental study of alpha-chymotrypsin-Sepharose
Biochem. Biophys. Res. Comm., 45 , 615-621 (1971)
V. KASCHE
Specific protein-protein interaction and its application in studies in radiation-induced protein modification
I. Interaction of a-chymotrypsin with soybean trypsin inhibitor. II. Interaction of a-chymotrypsin with soybean trypsin inhibitor bound to a polysaccharide matrix. III. Radiation-induced modification of a-chymotrypsin. Acta Universitatis Upsaliensis, 2 , 1-132 (1971)
V. KASCHE
Activity distributions in modified alpha-chymotrypsin
Biochem. Biophys. Res. Comm., 38 , 875-881 (1970)
V. KASCHE
Production and purification of 11C- and 13N-labelled alpha-chymotrypsin
Radiochem. Radioanal. Letters, 3 , 51-56 (1970)
H. LINSCHITZ, D.R. CROSS, V.KASCHE, and J. TENENBAUM
Low-temperature studies on phytochrome, light and dark reactions in the red to far-red transformation and intermediate forms of phytochrome
Proc. U.S. Nat. Acad. Sci., 61 , 1095-11o1 (1968)
H. LINSCHITZ, and V. KASCHE
Kinetics of phytochrome conversion. Multiple pathways in the PR to PFR reaction, as studied by double flash-technique
Proc. U.S. Nat. Acad. Sci., 58 , 1059-1064 (1967)
V.KASCHE
Radical intermediates in the fluorescein- and eosin- photosensitized autoxidation of 1-tyrosine
Photochem. Photobiol., 6 , 643-650 (1967)
H. LINSCHITZ, V.KASCHE, W.L. BUTLER, and H.W. Siegelmann
The kinetics of phytochrome conversion
J. Biol. Chem., 241 , 3395-3403 (1966)
V.KASCHE, and L. LINDQVIST
Transient species in the photochemistry of eosin
Photochem. Photobiol., 4 , 923-933 (1965)
V. KASCHE, and L. LINDQVIST
Reactions between the triplet state of fluorescein and oxygen
J. Phys. Chem., 68 , 817-823 (1964)

Other publications

V. KASCHE
Enzymtechnische Produktionsverfahren – können sie mit Gentechnik nachhaltiger gestaltet werden?
Chancen und Risiken der Gentechnik im Umweltschutz. Thüringer Ministeriumn für Umweltschutz, Erfurt, 1998, S 166-174
V. KASCHE
ENZYME ALS BIOKATALYSATOREN – NACHHALTIGE TECHNIK ZUR RESSOURCEN- UND UMWELTSCHONENDEN STOFFUMWANDLUNGEN FÜR DIE BEDARFSDECKUNG MIT MATERIELLEN GÜTERN ?
Simone Leinkauf Hrsg. Metropolis Verlag Marburg, 1997
V. KASCHE
Sensoren für die Biotechnik ?
Chemische Industrie, April 1991, 55-59
K. BUCHHOLZ, B. Gï¿½RLITZ, F. HEYFELD, V. KASCHE, M. KIPER und W. van den Daele
Technischer Fortschritt und fortschrittliche Technik. Wie nützlich ist die Bio-/Gen-Technologie”
Bericht einer Studiengruppe des Vereins deutscher Wissenschaftler – im Auftrage des BMFT, 1985
V. KASCHE
Research on enzyme and microbial technology in the Peoples Republic of China”
Enzyme Microb. Technol., 4, 125-126 (1982)
V. KASCHE
Biotechnische Entwicklungen zur Energiegewinnung und Stoffumwandlung”
Mitteilungen des Verbandes deutscher Biologen Nr. 253 (1979) (Beilage zu: Naturwiss. Rundschauf, 3 (1979)
V. KASCHE, and J. KRAFT
Framtidsforskningen och politiken”
Liberal Debatt (Sweden), 22, 5-8 (1979)
V. KASCHE
Molekulare Evolution und die Entstehung lebender Systeme – Zufall oder zwingende Notwendigkeit?”
Jahrbuch der Wittheit zu Bremen. Hrsg.: Die Wittheit zu Bremen, Bremen (1976)
V. KASCHE
Die fachsystematischen Aspekte bei der Biologielehrer – Ausbildung im Rahmen des Bremer Modells”
Biologie und currikulare Forschung (D. Rodi, Hrsg.) Aulis Verlag Deubner & Co., Köln, 36-42 (1975)
V. KASCHE, H. AMNEUS, and K.J. JOHANSSON
Den blï¿½ elektonen (The blue electron)”
Forskning och Framsteg (Sweden) 8, 30 (1973)

Books & book chapters

K. Buchholz, V. Kasche, U.T. Bornscheuer
Biocatalysts and enzyme technology
Wiley-VcH, Weinheim. 2005
K. Buchholz, V. Kasche
Biokatalysatoren und Enzymtechnologie
VCH, Weinheim, 1997

Patents

angemeldet (2002)
Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung
5,326,698 (1994)
Method of purification of penicillin amidase using phenylbutylamine-eupergit
GB 2 230 010 B ï¿½ continuation (1990)
Carrier comprising phenylbutylamine groups for purification or immobillisation of proteins
DE 39 09 018 A1 (1990)
Protein-Reinigungsverfahren
DE 35 07 403 C 2 (1986)
Enzymatische Semisynthese von ß-Lactamantibiotika